abstract：:A chimeric gene consisting of the 5' flanking sequences of a rice glutelin gene (Gt3) linked to the chloramphenicol acetyltransferase (CAT) coding segment was introduced into tobacco via Agrobacterium tumefaciens-mediated transformation. CAT enzyme activity could be detected in extracts from seeds as early as 8 days a...

journal_title：Plant molecular biology

authors： Leisy DJ,Hnilo J,Zhao Y,Okita TW

更新日期：1990-01-01 00:00:00

abstract：:Differential screening of a cDNA bank constructed from ripe tomato fruit mRNA allowed the isolation of cDNA clone 2A11 which is entirely fruit-specific, is expressed at steadily increasing levels from anthesis to breaker, and accounts for approximately 1% of the messenger RNA in mature tomato fruit. A genomic clone co...

journal_title：Plant molecular biology

authors： Pear JR,Ridge N,Rasmussen R,Rose RE,Houck CM

更新日期：1989-12-01 00:00:00

abstract：:Left- and right-border repeats, which surround the T-region, contain two conserved regions separated by 5 bp that are not conserved. At the onset of T-DNA processing virD-encoded proteins introduce a nick in the largest of these conserved regions (12 bp) at a specific position in the bottom strand between a guanine an...

journal_title：Plant molecular biology

authors： van Haaren MJ,Sedee NJ,de Boer HA,Schilperoort RA,Hooykaas PJ

更新日期：1989-11-01 00:00:00

abstract：:Anaerobiosis rapidly induces alcohol dehydrogenase (ADH), an enzyme of the fermentation pathway, in different parts of rice seedlings. After initiation of anaerobiosis, the activity of the enzyme increases linearly for 3 days or more. The ADH activity is anaerobically inducible even in mature rice leaves in contrast t...

journal_title：Plant molecular biology

authors： Xie Y,Wu R

更新日期：1989-07-01 00:00:00

abstract：:We have cloned and sequenced a full-length cDNA from barley (Hordeum vulgare L.) seeds encoding the bifunctional α-amylase/subtilisin inhibitor (BASI). The nucleotide sequence predicts an open reading frame coding for a protein of 203 amino acids. The first 22 amino acids exhibit the sequence characteristic of a signa...

journal_title：Plant molecular biology

authors： Leah R,Mundy J

更新日期：1989-06-01 00:00:00

abstract：:In this paper we report on the organization and expression of the two chalcone flavanone isomerase (CHI) genes A and B from thePetunia hybrida inbred line V30. From a combination of sequence data, primer extension and RNAse protection experiments we infer the presence of two promoters PA1 and PA2 upstream of the CHI g...

journal_title：Plant molecular biology

authors： van Tunen AJ,Hartman SA,Mur LA,Mol JN

更新日期：1989-05-01 00:00:00

abstract：:We have analyzed the nucleotide sequence and accumulation of an mRNA which is prevalent in seeds of Brassica napus L. During normal development, the mRNA begins to accumulate during late embryogeny, is stored in dry seeds, and becomes undetectable in seedlings within 24 hours after imbibition. Moreover, abscisic acid ...

journal_title：Plant molecular biology

authors： Harada JJ,Delisle AJ,Baden CS,Crouch ML

更新日期：1989-04-01 00:00:00

abstract：:Electrophoretic analysis of translation products of polyadenylated RNA isolated from mid-maturation sorghum seed in the presence of [(35)S]met, [(3)H]leu, or [(3)H]val revealed two major proteins of kDa and 21 kDa. These products were not detected when [(3)H]lys was supplied as the radioactive substrate. Under similar...

journal_title：Plant molecular biology

authors： Derose RT,Ma DP,Kwon IS,Hasnain SE,Klassy RC,Hall TC

更新日期：1989-03-01 00:00:00

abstract：:We have analysed the expression of the 8-10 members of the gene family encoding the flavonoid biosynthetic enzyme chalcone synthase (CHS) from Petunia hybrida. During normal plant development only two members of the gene family (CHS-A and CHS-J) are expressed. Their expression is restricted to floral tissues mainly. A...

journal_title：Plant molecular biology

authors： Koes RE,Spelt CE,Mol JN

更新日期：1989-02-01 00:00:00

abstract：:Green cybrids with a new nucleus-chloroplast combination cannot be selected after protoplast fusion in the intersubfamilial Nicotiana-Solanum combination. As an approach to overcome the supposed plastomegenome incompatibility, a partial plastome transfer by genetic recombination has been considered. After fusions of p...

journal_title：Plant molecular biology

authors： Thanh ND,Medgyesy P

更新日期：1989-01-01 00:00:00

abstract：:In this report, we describe the efficient expression and glycosylation, in insect cells, of β-phaseolin polypeptides (M r 45 and 48 kDa) from Phaseolus vulgaris, by means of a baculovirus expression vector. N-terminal sequence analysis demonstrated that the signal peptide was efficiently processed. Tunicamycin treatme...

journal_title：Plant molecular biology

authors： Bustos MM,Luckow VA,Griffing LR,Summers MD,Hall TC

更新日期：1988-11-01 00:00:00

abstract：:Plant infections with cassava latent virus (CLV) were mediated by the Ti plasmid of Agrobacterium tumefaciens containing either monomeric or dimeric copies of the virus genome. The CLV DNAs caused typical symptoms when they were inoculated in Agrobacterium strains C58, LBA4404 and a virE mutant A1026, but not other Ag...

journal_title：Plant molecular biology

authors： Morris BA,Richardson KA,Andersen MT,Gardner RC

更新日期：1988-11-01 00:00:00

abstract：:Cab-1 is a complex genetic locus in tomato consisting of four clustered genes encoding chlorophyll a/b-binding polypeptide. Southern blot analysis of total tomato DNA with genomic clones corresponding to the Cab-1 locus has revealed the presence of a repetitive element in the 3 kb spacer regions between two of these g...

journal_title：Plant molecular biology

authors： Bernatzky R,Pichersky E,Malik VS,Tanksley SD

更新日期：1988-09-01 00:00:00

abstract：:The chromatin structure of pea (Pisum sativum) rbcS genes in inactive (root), potentially active (dark-grown leaf), and active states (light-grown leaf) was analysed using (a) pancreatic DNAseI to detect general DNAseI sensitivity and DNAseI-hypersensitive sites, and (b) methyl-sensitive restriction endonucleases to p...

journal_title：Plant molecular biology

authors： Görz A,Schäfer W,Hirasawa E,Kahl G

更新日期：1988-09-01 00:00:00

abstract：:The actin gene superfamily of Petunia hybrida cv. Mitchell contains greater than 100 gene members which have been divided into several highly divergent subfamilies [1]. Five subfamily-specific probes have been used to compare the actin genes among the Mitchell, Violet 23 (V23) and Red 51 (R51) cultivars of P. hybrida....

journal_title：Plant molecular biology

authors： McLean M,Baird WV,Gerats AG,Meagher RB

更新日期：1988-09-01 00:00:00

abstract：:The nucleotide sequence for the Photosystem II gene psbC has been determined for the cyanobacterium Synechocystis 6803. The gene overlaps the last 50 bases of the psbD gene, and both genes are transcribed in the same direction, but read in different frames. This arrangement is identical to that found in all chloroplas...

journal_title：Plant molecular biology

authors： Chisholm D,Williams JG

更新日期：1988-07-01 00:00:00

abstract：:The development of an efficient transformation system is a prerequisite for the molecular analysis of gene expression in plants. In crop plants, this development has been hindered by difficulties encountered both in whole plant regeneration from protoplasts and in the general insusceptibility of monocots to Agrobacter...

journal_title：Plant molecular biology

authors： Wang YC,Klein TM,Fromm M,Cao J,Sanford JC,Wu R

更新日期：1988-07-01 00:00:00

abstract：:As a highly active plant viral promoter that is able to function in a wide variety of cell types, the cauliflower mosaic virus (CaMV) 35S promoter has the potential for harboring a plant enhancer element. We tested this possibility and demonstrated that a 338 base pair fragment isolated from the region upstream of the...

journal_title：Plant molecular biology

authors： Odell JT,Knowlton S,Lin W,Mauvais CJ

更新日期：1988-05-01 00:00:00

abstract：:Antisera against the N-terminal and C-terminal parts of the potential ORF IV product were used to analyse extracts from CaMV-infected turnip leaves by immunoblotting. Polypeptides of 87, 83, 82, 60 and 57 kDa were detected. The origin of these proteins is discussed. ...

journal_title：Plant molecular biology

authors： Kirchherr D,Albrecht H,Mesnard JM,Lebeurier G

更新日期：1988-05-01 00:00:00

abstract：:Bacteroids of Rhizobium leguminosarum in root nodules of Pisum sativum are enclosed by a plant-derived peribacteriod membrane (PBM). The contents of the interstitial peribacteroid space (PBS) between bacteroid membrane and PBM were isolated by a controlled osmotic shock of PBM-enclosed bacteroids and analysed by two-d...

journal_title：Plant molecular biology

authors： Katinakis P,Lankhorst RM,Louwerse J,van Kammen A,van den Bos RC

更新日期：1988-03-01 00:00:00

abstract：:Tomato golden mosaic virus (TGMV), a member of the geminivirus group, has a genome consisting of two DNA molecules designated the A and B components. Both are required for infectivity in healthy plants, although the former has been shown to replicate independently in transgenic plants containing tandem direct repeats ...

journal_title：Plant molecular biology

authors： Sunter G,Gardiner WE,Rushing AE,Rogers SG,Bisaro DM

更新日期：1987-11-01 00:00:00

abstract：:The identification of some nodule-specific host proteins (nodulins) from common bean (Phaseolus vulgaris L.), a tropical ureide-transporting legume, is described. Particularly, the existence and developmental expression of several abundant nodule-specific transcripts of P. vulgaris are shown, including leghemoglobin, ...

journal_title：Plant molecular biology

authors： Campos F,Padilla J,Vázquez M,Ortega JL,Enríquez C,Sánchez F

更新日期：1987-11-01 00:00:00

abstract：:Tomato protoplasts have been transformed with plasmid DNA's, containing a chimeric kanamycin resistance gene and putative tomato origins of replication. A calcium phosphate-DNA mediated transformation procedure was employed in combination with either polyethylene glycol or polyvinyl alcohol. There were no indications ...

journal_title：Plant molecular biology

authors： Jongsma M,Koornneef M,Zabel P,Hille J

更新日期：1987-09-01 00:00:00

abstract：:The High Mobility Group (HMG) proteins of vertebrate animals have been the subject of intensive study because of evidence that they may be structural proteins of transcriptionally active chromatin. Organisms other than vertebrate animals have chromatin proteins which meet the operational criteria of salt extractabilit...

journal_title：Plant molecular biology

authors： Spiker S,Everett KM

更新日期：1987-09-01 00:00:00

abstract：:Many chloroplast proteins are synthesized in the cytoplasm as precursors which contain an amino terminal transit peptide. These precursors are subsequently imported into chloroplast and targeted to one of several organellar locations. This import is mediated by the transit peptide, which is cleaved off during import. ...

journal_title：Plant molecular biology

authors： Smeekens S,van Steeg H,Bauerle C,Bettenbroek H,Keegstra K,Weisbeek P

更新日期：1987-07-01 00:00:00

abstract：:A method is described for the high frequency transformation of carrot proembryogenic suspension culture cells by a non-oncogenic Ti-plasmid vector (pGV3850::1103) which carried a chimaeric kanamycin resistance gene (nos-NPT-II). Plants were regenerated efficiently from transformed material by somatic embryogenesis in ...

journal_title：Plant molecular biology

authors： Scott RJ,Draper J

更新日期：1987-05-01 00:00:00

abstract：:Three polypeptides of 33 kD, 23 kD and 16 kD are released from maize photosystem II particles by alkaline Tris solution treatment and shown to cross-react with antisera to purified spinach oxygen-evolving (OE) polypeptides of 34 kD, 23 kD and 17 kD, respectively. They are not located exclusively in mesophyll cells but...

journal_title：Plant molecular biology

authors： Sheen JY,Sayre RT,Bogorad L

更新日期：1987-05-01 00:00:00

abstract：:The chloroplast gene for the epsilon subunit (atpE) of the CF1/CF0 ATPase in the green alga Chlamydomonas reinhardtii has been localized and sequenced. In contrast to higher plants, the atpE gene does not lie at the 3' end of the beta subunit (atpB) gene in the chloroplast genome of C. reinhardtii, but is located at a...

journal_title：Plant molecular biology

authors： Woessner JP,Gillham NW,Boynton JE

更新日期：1987-03-01 00:00:00

abstract：:The photosynthetic apparatus of plant chloroplasts contains two photosystems, termed Photosystem I (PSI) and Photosystem II (PSII). Both PSI and PSII contain several types of chlorophyll a/b-binding (CAB) polypeptides, at least some of which are structurally related. It has been previously shown that multiple genes en...

journal_title：Plant molecular biology

authors： Pichersky E,Hoffman NE,Malik VS,Bernatzky R,Tanksley SD,Szabo L,Cashmore AR

更新日期：1987-03-01 00:00:00

abstract：:Using a 2,4-dichlorophenoxyacetic acid (2,4-D)-dependent tobacco cell-suspension culture we analyzed early hormone-induced molecular events preceding cell division. By differential screening of a cDNA library to mRNAs derived from hormone-starved cells treated with 2,4-D for 4 h, seven non-cross hybridizing cDNA clone...

journal_title：Plant molecular biology

authors： van der Zaal EJ,Memelink J,Mennes AM,Quint A,Libbenga KR

更新日期：1987-03-01 00:00:00

abstract：:The treatment of barley aleurone layers with gibberellic acid (GA3) results in the synthesis of two groups of α-amylase isozymes. Addition of abscisic acid (ABA) at the same time as GA3 inhibited the synthesis of both groups of isozymes. However, midcourse ABA addition (12 h or later after GA3) had a more inhibitory e...

journal_title：Plant molecular biology

authors： Nolan RC,Lin LS,Ho TH

更新日期：1987-01-01 00:00:00

abstract：:The effect of red light and far-red light on the appearance of mRNA for the small and large subunit of ribulose-1,5-bisphosphate carboxylase in rye seedlings has been analysed. Irradiation of etiolated seedlings with a pulse of red light increased the mRNA level of the small subunit by a factor of 7-10 and that of the...

journal_title：Plant molecular biology

authors： Ernst D,Pfeiffer F,Schefbeck K,Weyrauch C,Oesterhelt D

更新日期：1987-01-01 00:00:00

abstract：:The nucleotide sequence distal to the rRNA operon from maize chloroplasts has been analyzed. It contains genes coding for tRNA(Arg)(ACG) and tRNA(Asn)(GUU). The tRNA(Arg)(ACG) gene, which is separated from the last rRNA gene of the rRNA operon, the 5S rDNA, by an intergenic region of 252 bp, has the same orientation a...

journal_title：Plant molecular biology

authors： Dormann-Przybyl D,Strittmatter G,Kössel H

更新日期：1986-11-01 00:00:00

abstract：:A highly purified RNA polymerase preparation from pea chloroplasts has been shown to specifically transcribe the 16S rRNA gene in vitro using the recombinant pCB2-8 DNA as a template. The RNA polymerase has been found to show maximum activity and specificity with pea supercoiled rDNA as a template. At low concentratio...

journal_title：Plant molecular biology

authors： Sun E,Shapiro DR,Wu BW,Tewari KK

更新日期：1986-11-01 00:00:00

abstract：:A hybrid Cauliflower Mosaic Virus (CaMV) genome containing a selectable marker gene was constructed by replacing the gene VI coding region with the aminoglycoside (neomycin) phosphotransferase type II [APH(3')II] gene from Tn5. This modified viral genome was tested for its infectivity both in planta and in a protoplas...

journal_title：Plant molecular biology

authors： Paszkowski J,Pisan B,Shillito RD,Hohn T,Hohn B,Potrykus I

更新日期：1986-09-01 00:00:00

abstract：:Transformed clones from a shooty tobacco crown gall tumor, induced byAgrobacterium tumefaciens strain LBA1501, having the auxin locus of the TL-region inactivated by a Tn1831 insertion, were investigated for their T-DNA structure and expression. It has been described previously (28) that in addition to clones with an ...

journal_title：Plant molecular biology

authors： Peerbolte R,Leenhouts K,Hooykaas-van Slogteren GM,Wullems GJ,Schilperoort RA

更新日期：1986-07-01 00:00:00

abstract：:Two dimensional gel electrophoresis was used to examine differences in steady state total poly(A) RNA from untransformed potato (Solanum tuberosum cv. Maris Bard) and potato transformed with shoot-inducing TL-DNA from A. tumefaciens. RNA was compared from phenotypically very distinct in vitro cultured shoots, more sim...

journal_title：Plant molecular biology

authors： Burrell MM,Temple S,Ooms G

更新日期：1986-07-01 00:00:00

abstract：:The expression of chloroplast genes during cotton embryogenesis was followed by measuring hybridization of cloned chloroplast genomic fragments to total RNA immobilzed on membrane filters. RNA was extracted from the cotyledons of cotton embryos at different stages of development ranging from very small embryos (5 mg) ...

journal_title：Plant molecular biology

authors： Borroto KE,Dure L 3rd

更新日期：1986-03-01 00:00:00

abstract：:Ribulose bisphosphate carboxylase-oxygenase (Rubisco) is a key enzyme in the photosynthetic fixation of CO2 by the chloroplast. The synthesis of the enzyme is an example of the cooperation between the chloroplast and the nucleocytoplasmic compartments, as it is assembled from subunits encoded in the two respective gen...

journal_title：Plant molecular biology

authors： Goldschmidt-Clermont M

更新日期：1986-01-01 00:00:00

abstract：:The relative amounts of some chloroplast polypeptides were followed during greening of leaves from three different plant families. Oat, bean and spinach were the representatives of the Gramineae, Leguminosae and Chenopodiaceae, respectively. By using specific antibodies against subunits of the chloroplast protein comp...

journal_title：Plant molecular biology

authors： Nechushtai R,Nelson N

更新日期：1985-11-01 00:00:00